Figure 1.

Scheme of vectors used for protoplast transient transfections in this study. (A) The protein-fusion reporter vectors. The coding sequences of wild-type and mutant Jas-motif and the JasSHY chimeric protein were fused in-frame to the N-terminus of firefly luciferase (fLuc) reporter gene. Expression of reporter proteins were under the control of the UBQ10 promoter. (B) The effector vectors. Expression of SHY2, JaSHY chimera, COI1 and COI1 mutant effector proteins were placed under the control of the UBQ10 promoter. All reporter and effector proteins contain a hemagglutinin (HA) epitope tag at the N-terminus. (C) The promoter-reporter vector. The auxin responsive DR5 promoter sequence was fused to the firefly luciferase (fLuc) reporter gene. Not drawn to scale.