Figure 2.

The Jas-motif of JAZ1 is sufficient to target fLuc for degradation. (A) Schematic and amino acid residues of JAZ1 Jas-motif. The red bar indicates residues of the Jas-motif with the sequence shown below. The red letters highlight wild-type residues and point mutations in Jas and Jas^AA, respectively. (B) Arabidopsis leaf protoplasts prepared from coi1 mutant plants were co-transfected with protein-fusion reporter plasmids encoding Jas-fLuc or Jas^AA-fLuc under the control of UBQ10 promoter and effector plasmid encoding COI1 under the control of UBQ10 promoter. Firefly luciferase (fLuc) activities were normalized to Renilla luciferase (rLuc) activities. Reporter activities of Jas-fLuc without effector vector were set to one. Values represent means (±SE) of four independently transformed batches of protoplasts.