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. 2020 Mar 6;9(3):641. doi: 10.3390/cells9030641

Figure 4.

Figure 4

The ARF7 transcriptional activity was released upon JaSHY degradation by COI1. Arabidopsis leaf protoplasts prepared from coi1 mutant plants were co-transfected with promoter-driven reporter plasmid DR5pro:fLuc and effector plasmids encoding ARF7, SHY2, JaSHY, or COI1 under the control of UBQ10 promoter. Firefly luciferase (fLuc) activities were normalized to Renilla luciferase (rLuc) activities. Reporter activities of fLuc without effector vector were set to one. Values represent means (±SE) of four independently transformed batches of protoplasts.