Figure 4.

Rearrangement of E-cadherin-based AJs in parallel with reorganization of the actin cytoskeleton in IAR-20 cells treated with EGF. (a) Immunostaining of E-cadherin and β-actin. Top—red and green channels merged. The cell-cell boundaries enlarged below are indicated with arrows. Disorganization of AJs begins 5 min after addition of EGF, simultaneously with dissolution of the circumferential actin bundle and formation of lamellipodia. Beginning from 10–15 min after the addition of EGF, punctate AJs were connected with straight actin fibers appear. (b) Fluorescence intensity profiles of F-actin and β-catenin in the zones of cell-cell contacts. N = 30 for every graph.