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. 2020 Mar 6;9(3):639. doi: 10.3390/cells9030639

Figure 5.

Figure 5

Downregulation of miR-1246, miR-182, and miR-486 promotes PASMC migration. (A). PASMCs transfected with control or anti-miRNAs were subjected to the scratch wound assay in presence or absence of PDGF-BB. The distance of the migration was measured using ImageJ at 8 h after a scratch wound was introduced. (B). The quantification graph of the relative migration distance. The means ± S.E. of triplicate measurements of three independent experiments are shown; * p < 0.05. (C). Levels of miRNAs relative to U6 snRNA measured by qRT-PCR in PASMCs transfected with control or anti-miRs. Data represent the means ± S.E. of triplicates. * p < 0.05. (D). PASMCs transfected with control or miRNA mimics were subjected to the scratch wound assay in the presence or absence of PDGF-BB. The distance of the migration was measured using ImageJ at 8 h after a scratch wound was introduced. (E). The quantification graph of the relative migration distance. The means ± S.E. of triplicate measurements of three independent experiments are shown; * p < 0.05. (F). Levels of miRNAs relative to U6 snRNA measured by qRT-PCR in PASMCs transfected with control or miRNA mimics. Data represent the means ± S.E. of triplicates. * p < 0.05.