Figure 4.

Expression of CD127 by CD4⁺FOXP3⁺ cells from the bone marrow and peripheral blood of OA and RA patients. (a) Representative dot plots show the expression of CD127 and CD25 by gated CD4⁺ T cells in OA and RA BM and peripheral blood. (b) The percentage of CD127⁻CD25⁺ among CD4⁺ T cells from the BM and peripheral blood of the same patient (n = 6). Individual results are shown as dots. Comparison of the BM with the blood from the same patient (done separately for OA and RA patients) was analyzed by the Wilcoxon test. (c) Representative dot plots show CD127 and CD127 together with CD25 expression by gated CD4⁺FOXP3⁺ cells in the BM and peripheral blood of OA and RA patients. (d) The percentage of CD127⁺ cells among CD4⁺FOXP3⁺ T cells from the BM of OA and RA patients (chart on the left), and the BM and peripheral blood of the same patient (RA—chart in the middle, OA—chart on the right; n = 6). Data on the left chart are presented as median with a min–max range, and differences between groups of patients were analyzed by Mann–Whitney U-test. On the remaining two charts, individual results are shown as dots, and a comparison of the BM with the blood from the same patient was analyzed by the Wilcoxon test (done separately for OA and RA patients). Numbers depicted on dot plots show the frequencies of subset expressing a proper marker. OA/RA BM/blood—cells isolated from the BM/peripheral blood of patients with OA/RA, respectively.