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. 2020 Feb 27;9(3):559. doi: 10.3390/cells9030559

Figure 2.

Figure 2

Promoter hypermethylation mediates downregulation of UCHL1 in nasopharyngeal carcinoma. (A,B) qRT-PCR analysis of UCHL1 mRNA expression in normal nasopharyngeal epithelial cell lines (NP69 and N2-Tert) and nasopharyngeal carcinoma cell lines (5-8F, CNE2, HNE1, SUNE1, 6-10B, HONE1) (A), in normal (n = 12) and NPC (n = 15) tissues (B). (C,D) Immunoblot analysis of UCHL1 and α-tubulin protein expression in NP69 and nasopharyngeal carcinoma cell lines (C), in normal (N, n = 5) and NPC (T, n = 5) tissues (D). (E) UCHL1 methylation levels determined by bisulfite pyrosequencing analysis in NP69 and nasopharyngeal carcinoma cell lines treated with or without DAC. (F) Quantitative RT-PCR analysis of UCHL1 mRNA expression in NP69 and nasopharyngeal carcinoma cell lines treated with or without DAC. * p < 0.05; *** p < 0.001; Mean ± S.D.; Student’s t-test. Data are representative of at least three independent experiments.