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. 2020 Feb 27;9(3):559. doi: 10.3390/cells9030559

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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UCHL1 inhibits the migration and invasion of NPC cells in vitro. (A) qRT-PCR analysis of UCHL1 mRNA (up) and immunoblot analysis of UCHL1 and α-tubulin (down) in SUNE1 and HONE1 cells transfected with empty vector or plasmid encoding UCHL1. (B,C) Cell migration was measured using a wound healing assay (×200) (B) and transwell assay (×200) without Matrigel (C). Invasion was measured using a transwell assay with Matrigel (×200) (C). (D) qRT-PCR analysis of UCHL1 mRNA (up) and immunoblot analysis of UCHL1 and α-tubulin (down) in SUNE1 and HONE1 cells transfected with control or shUCHL1(#1 and #2) that stably overexpressed UCHL1. (E–H) Cell migration was measured using a wound healing assay (E) and transwell assay without Matrigel (F,G). Invasion was measured using a transwell assay with Matrigel (F,H). Scale bar:100 µm; Mean ± S.D.; * p < 0.05, ** p < 0.01; *** p < 0.001; Student’s t-tests. Data are representative of at least three independent experiments.