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Co-culture of trigeminal ganglia and human AB10 ameloblastoma cells. (A) Schematic representation of the experimental set-up. (B) Overview of axons crossing the microgrooves and entering the AB10-containing culture chamber. (C–F) Brightfield images showing directional growth of axons towards AB10 cells at different distances from the chamber’s borders. Black arrowheads highlight axons growing towards AB10 cells. Scale bars: (B): 500 µm; (C–E): 100 µm.
