Figure 6.

(a) Illustration of the experimental procedure of Raman imaging of the equatorial plane of a RBC hold by four infrared (1064 nm) optical traps by a Raman excitation probe (532 nm) with scanning step-size of 0.5 $\mathsf{\mu }$m. (b) Reconstructed hemoglobin distribution within healthy (left) and thalassemic (right) RBCs in cell equatorial plane. Normal hemoglobin distribution presents classical ring-shape, whereas the possibility to detect hemoglobin within the central area increases in diseased RBC [113].