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. 2020 Mar 6;9(3):635. doi: 10.3390/cells9030635

Figure 2.

Figure 2

Analysis of the contents of lymphocytes in CAISMOV24 cell aggregates obtained by 3D co-culture of CAISMOV24 cells with PBMCs. (a) 3D co-cultures performed with NanoShuttle-conjugated PBMCs (n = 6 experimental repetitions) have significant (* p < 0,05) higher percentages of CD3 lymphocyte subset than co-cultures performed with PBMCs non-conjugated with NanoShuttle (n = 3 experimental repetitions). (b) Contour plots from a representative assay performed with NanoShuttle-conjugated and non-conjugated PBMCs from the same blood sample. (c) Fluorescence microscopy from a representative cryo-section of cell aggregates showing CFSE-labelled PBMCs (arrow). (d) The ratio of lymphocytes retrieved from cell aggregates is similar between co-cultures at 72 h that were supplemented or not with IL-15 (n = 7 experimental repetitions).