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. 2020 Mar 6;9(3):635. doi: 10.3390/cells9030635

Figure 3.

Figure 3

Comparison of the contents of lymphocyte subsets in CAISMOV24 cell aggregates obtained by 3D co-culture of CAISMOV24 cells with PBMCs. (a), (b) and (c) frequencies of T lymphocytes subsets T-CD4, T-CD8 and T-reg respectively, as well as (d) NK lymphocytes. (b) T-CD8+ subset is significant lower (p < 0.05) in CAISMOV24 cell aggregates supplemented with IL-15 compared with PBMCs maintained in the same culture conditions. (c) T-reg subset is significantly higher (p < 0.05) in CAISMOV24 cell aggregates supplemented with IL-15 compared to PBMCs cultures. CD69 molecule is upregulated on NK and CD8 lymphocytes retrieved from CAISMOV24 cell aggregates. (e,f) CD69 upregulation on CD8+ lymphocytes is associated with supplementation of the culture with IL-15, while (g,h) on NK lymphocytes it was associated with presence of CAISMOV24 cells. Values were presented as whisker plots and medians (n = 7 experimental repetitions with different blood donors); statistical analyses were performed by ANOVA followed by Tukey’s multiple comparisons test. Significant statistical differences are indicated with * (* p < 0.05, **p < 0.01 and *** p < 0.001).