Figure 1.

Relationship between Ca²⁺-dependent characteristics of the TMEM16A/anoctamin1 (ANO1) current and voltage-gated Ca²⁺ channel (VGCC). (A) Rod bipolar cells were stimulated from a holding potential of −70 mV to a membrane potential 10 mV for 250 ms. Representative current traces before (gray) and 300 s after drug administration (black, blue, pink). The current area was normalized by the area of I_tail at 150 s, and the normalized current area over time (right) showed the inhibitory effect of ANO1-specific blockers (n = 7; p < 0.05, Student’s t-test). (B) Internal application of 5 mM BAPTA (black; 0.56 ± 0.03) decreased I_tail compared with the control (gray; 0.21 ± 0.06; n = 7; p < 0.05, Student’s t-test). (C) Rod bipolar cells were stimulated from a holding potential of −70 to −60 mV until approximately 20 mV. Current traces (left) and the normalized current area of I_tail (right) against command voltage were plotted. (D) The current area was normalized by the area of I_tail at 200 s, and the normalized current area over time showed the inhibitory effects of L- and T-type VGCC inhibitors, namely 40 μM nifedipine and 40 μM mibefradil, respectively (n = 7; ANOVA, p < 0.05).