Figure 4.

Effect of dominant negative TRPC5 (DN-TRPC5) on phosphatidylserine (PS) externalization in HEK293 cells. (A–C) Representative images showing DN-TRPC5-mCherry expression and PS externalization on the plasma membrane of HEK293 cell transfected with DN-TRPC5-mCherry+PLSCR1. The cells were treated with saline (control) (A), hypotonic solution (B), or LaCl₃ (100 μmol/L) (C). (D) Summary data showing the PS externalized cells (FITC-positive) in percentage of total cells (%) in A–C. (E) Combined summary data showing the PS externalized cells (FITC-positive) in percentage of total cells (%) from Figure 2, Figure 3 and Figure 4. The data were from FITC channel. PS externalization was detected as green fluorescence via the annexin V-FITC assay. DN-TRPC5-mCherry was detected as red fluorescence. Values are shown as the mean ± SEM (n = 3); *p < 0.05 for Control vs. Hypotonic or LaCl₃ with a two-tailed unpaired Student’s t test; ^#p < 0.05, PLSCR1+TRPC5 vs. vector, PLSCR1, TRPC5 or DN-TRPC5+PLSCR1 with two-way ANOVA followed by the Tukey’s post-hoc comparison test.