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. 2020 Mar 4;9(3):620. doi: 10.3390/cells9030620

Table 1.

Sequence of primers used for quantitative real-time PCR.

Species Gene Forward Primer Reverse Primer
Human 18S GAGGATGAGGTGGAACGTGT GGACCTGGCTGTATTTTCCA
GAPDH TGTCAAGCTCATTTCCTGGTA CACAGGGTACTTTATTGATGG
DBP CCAATCATGAAGAAGGCAAGAAA GGCTGCCTCGTTGTTCTTGT
PER2 GCTGGCCATCCACAAAAAGA GCGAAACCGAATGGGAGAAT
BMAL1 GAGAAGGTGGCCCAAAGAGG GGAGGCGTACTCGTGATGTT
NR3C1 AGCAGTGGAAGGTAGACAGC CCTGTAGTGGCCTGCTGAAT
CREB1 TTCTCCGGAACACAGATTTCA AATCCTTGGCACTCCTGGTG
CREB3 AGAGTGAGAGCTGTAGAAAAGAGG AATCTTCCTCCGCACACGTT
PRKACA AAGAAGGGCAGCGAGCAG CTGTGTTCTGAGCGGGACTT
PRKACB AGAGAACCACCTTGTAACCAGTA TGGCTTTGGCTAGAAACTCTT
CREBBP TGGCTGAGAACTTGCTGGAC TGGAAGCAGCATCTGGAACA
EP300 ACCAGGAATGACTTCTAGTTTGA TACGAGGCCCATAGCCCATA
CRTC1 CAGCCGAGGCCAGTACTATG AAGGGGGTCAGAGAGACAGG
CRTC2 GGTGATGATGGACATCGGCT CCGAGTGCTCCGAGATGAAT
CRTC3 TGTGGGTTTTGACCAGCAGT TCTTTGAACAGGCTGGTGCT
CLOCK ACGACGAGAACTTGGCATTG TCCGAGAAGAGGCAGAAGG
PER1 CCCAGCACCACTAAGCGTAAA TGCTGACGGCGGATCTTT
PER3 GCCTTACAAGCTGGTTTGCAA CTGTGTCTATGGACCGTCCATTT
CRY1 ACTCCCGTCTGTTTGTGATTCG GCTGCGTCTCGTTCCTTTCC
CRY2 TCTTCCAGCAGTTCTTCC GTAGTCCACACCAATGATG
CIRBP CAGATCTCTGAAGTGGTGGT CCTGCCTGGTCTACTCGGAT
HSF1 TGCAGCTGATGAAGGGGAAG AGGATCCGGTTTGACTGCAC
DDIT4 GGTTCGCACACCCATTCAAG CAGGGCGTTTGCTGATGAAC
TFRC GGACGCGCTAGTGTTCTTCT CATCTACTTGCCGAGCCAGG
VEGFA CCCTGATGAGATCGAGTACAT CGGCTTGTCACATCTGCAAGT
EGLN1 AGCCCAGTTTGCTGACATTG TCGTGCTCTCTCATCTGCATC
HIF1A CCTCTGGACTTGCCTTTCCT TGGCTGCATCTCGAGACTTT
Mouse 18s GAGGATGAGGTGGAGCGAGT GAACCTGGCTGTACTTCCCA
Dbp CGTGGAGGTGCTTAATGACCTTT CATGGCCTGGAATGCTTGA
Per2 ATGCTCGCCATCCACAAGA GCGGAATCGAATGGGAGAAT
Bmal1 CCAAGAAAGTATGGACACAGACAAA GCATTCTTGATCCTTCCTTGGT