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. 2020 Mar 6;9(3):636. doi: 10.3390/cells9030636

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Differentiation of TMSCs after STC1 inhibition. Differentiation of TMSCs was evaluated by specific staining after STC1 inhibition (A–D). Osteogenic differentiation was induced in untreated or siCTL- and siSTC1-treated TMSCs and stained by Alizarin red S at day 10, 15, and 20, followed by the elution for quantification (A,B). Adipogenic differentiation was induces in TMSCs and stained with Oil Red O at day 10, 15, and 20, followed by the elution for quantification (C,D). Results are three technical replicates of TMSC from one donor. Representative results from two different TMSCs with similar tendency were presented. Results are shown as mean ± SD.