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. 2020 Mar 6;9(3):636. doi: 10.3390/cells9030636

Figure 6.

Figure 6

ROS regulation in THP1-derived macrophage-like cells by STC1. THP1-derived macrophage like cells were stimulated with LPS and ATP for NLRP3 activation and co-cultured with human TMSCs. IL-1β production was detected by ELISA (A–C). TMSCs were added to macrophages at LPS priming step (A) or at ATP stimulation step (B), and NLRP3 activation was assessed by IL-1β secretion. Untreated or siSTC1-treated TMSCs were co-cultured with THP1-derived macrophages from LPS priming step (C). After 24 h of co-culture, IL-1β secretion in the supernatants was quantified by ELISA (A–C). After 24 h of co-culture using transwell, mitochondrial ROS level was measured by staining with mitoSOX. mitoSOX+ cells were determined by flow cytometry and quantified (D) Results are three technical replicates of TMSCs from one donor. Representative results from two different TMSCs with similar tendency were presented. **P < 0.005, *** P < 0.001. Results are shown as mean ± SD.