Skip to main content
. 2020 Mar 5;9(3):632. doi: 10.3390/cells9030632

Figure 7.

Figure 7

MiR-361 binds to HSP90 mRNA and suppresses its expression. (A) Illustration of the predicted binding site between miR-361 and HSP90 mRNA, with mutant sites shown in red. (B) The mRNA expression of HSP90 in SiHa cells transfected with miR-361 mimic or control mimic. (C) The mRNA level of HSP90 in HeLa cells transfected with miR-361 inhibitor and control inhibitor. (D) Western blot analysis of HSP90 and GAPDH expression in cervical cancer cells transfected as indicated. Numbers denote fold change of the HSP90/GAPDH protein ratio in miR-361 mimic-transfected SiHa cells versus control mimic-transfected cells (set as default 1), or in miR-361 inhibitor-transfected HeLa cells versus control inhibitor-transfected cells (set as default 1). (E) Luciferase assays in SiHa cells transfected with wild-type (WT) or mutant (MUT) HSP90 3′-UTR, together with miR-361 mimic or control mimic. (F) Luciferase assays in HeLa cells transfected with WT or MUT HSP90 3′-UTR, together with miR-361 inhibitor or control inhibitor. * p < 0.05.