Fig. 5.

Consequences of split-gene misannotations. a Comparing expression estimates across homologs. For the correct split-gene annotations (Split Supported (SS) in Fig. 4), expression of each split-gene is compared to the one expression value from the single gene to which they corresponded. b Exemplar of differential expression misinference when two distinct genes are incorrectly annotated as one. Expression differences (between immature ear and anther) of component genes cancel out resulting in no differential expression for the single rightmost gene. c Example of misinference for differential exon usage. Incorrect annotation as a single gene in PH207 should be two genes (split at location demarcated with the red X) as annotated in W22. Colored lines indicate separate tissues. d Median p-value across the per-exon tests of differential exon usage for each gene. Inflation of low p-values is observed when distinct genes are incorrectly treated as a single, merged gene (Merged is not supported (MNS) in Fig. 4)