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. 2020 Mar 13;9(3):704. doi: 10.3390/cells9030704

Figure 1.

Figure 1

Effect of carbonic anhydrase III (CA III) on cell growth, motility, migration, and invasion in oral cancer cells. (A) Western blot of SCC-9 and SAS CA III stable clones, where β-actin was used as the internal control. (B) GFP and GFP-CA III expression were observed by fluorescence microscopy. (C) Growth curves of SCC-9 and SAS were analyzed by the MTT assay after the transfection of GFP or the GFP-CA III vector for 48 h. (D) SCC-9 and SAS CA III stable clones were wounded for 0, 12, and 24 h. Phase-contrast pictures of the wounds at three different locations were taken. (E) Migration ability of SCC-9 and SAS CA III stable clones were measured after 24 h. (F) Invasion ability of SCC-9 and SAS CA III stable clones were measured after 48 h. * p < 0.05 compared with GFP.