Figure 1.

TLR8 and TLR7/8, but not TLR7 ligands, induce and co-stimulate IFN-γ production in Vδ2 T cells. Peripheral blood mononuclear cells (PBMC) from healthy donors were stimulated for 6 (a) or 12 h (b) with 1 μg/mL Imiquimod (TLR7 ligand), 0.1 μg/mL TL8-506 (TLR8 ligand), or 1 μg/mL Resiquimod (TLR7/8 ligand) in the absence (upper panel) or presence (lower panel) of 10 nM HMBPP. Monensin was present during the last 4 h of stimulation. Thereafter, cells were surface stained with anti-CD3 and anti-Vδ2 mAb, and permeabilized before staining with anti-IFN-γ mAb. A gate was set on Vδ2-positive cells. Black lines indicate the controls (medium only in upper row, medium with HMBPP in lower row). Results are representative of four independent experiments.