Figure 4.

TLR8 ligands suppress the monocyte-dependent re-stimulation of Vγ9Vδ2 T-cell lines with bromohydrin pyrophosphate (BrHPP). 5 × 10⁴ γδ T-cell lines (>95% CD3⁺Vδ2⁺) cells were cultured in 50 IU/mL L-2 with (grey columns) or without (open columns) 5 × 10⁴ purified monocytes in the absence of presence of 300 nM BrHPP and Resiquimod (1 μg/mL), TL8-506 (0.1 μg/mL) or Motolimod (0.5 μg/mL) as indicated. The absolute number of viable Vγ9 T-cells was determined after 3 or 6/7 days by SCDA. All cultures were set up in duplicates. (a) and (b) Results (absolute cell numbers per well; mean of duplicates) of a representative experiment are shown for day 3 (a), and for day 6 (b). (c) Results with additional Vδ2 T-cell lines cultured without monocytes and with or without TLR ligands; (d) Results with the same Vδ2 T-cell lines in the additional presence of monocytes and BrHPP. In (c) and (d), cell numbers of the respective control (c: medium only; d: monocytes plus BrHPP) were set to 100%, and results are displayed as % of controls. c: Resiquimod (n = 5), TL8-506 (n = 2), Motolimod (n = 2). d: Resiquimod (n = 9), TL8-506 (n = 7), Motolimod (n = 6). **** p < 0.0001.