Figure 6.

TLR8 and TLR7/8 but not TLR7 ligands up-regulate PD-L1 expression on monocytes. Purified monocytes were cultured for 12 h in medium supplemented or not with 1 μg/mL Imiquimod, 0.1 μg/mL TL8-506, or 2 μg/mL Resiquimod. Thereafter, cells were stained with anti-PD-L1-BV421 (upper row) and anti-CD277-PE (lower row) as indicated. Open black histogram: staining of freshly isolated monocytes. Filled grey histograms: medium control after 12 h. Colored histograms: Staining after incubation with respective ligands. Results of one out of two experiment are shown.