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. 2020 Mar 17;9(3):735. doi: 10.3390/cells9030735

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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AQP4b and AQP4d are absent from orthogonal arrays of particles (OAPs) in isoosmotic and hypoosmotic conditions. (A) Structured illumination microscopy (SIM) micrographs of astrocytes in isoosmotic conditions overexpressing AQP4b or AQP4d. Green spots represent vesicles carrying recombinant AQP4b (A_i) and AQP4d (A_ii), and red spots represent OAPs labeled with NMO-IgG antibodies. Higher magnifications are shown in the insets. Scale bars: 10 µm (inset, 0.5 µm). (B) SIM micrographs of astrocytes before and after hypoosmotic stimulation. Green spots represent vesicles carrying recombinant AQP4b (A_i) and AQP4d (A_ii), and red spots represent OAPs labeled with NMO-IgG antibodies. Panels from left to right show astrocytes in isoosmotic conditions and 2 and 10 min after hypoosmotic stimulation. In hypoosmotic conditions, AQP4b and AQP4d isoforms remained absent from OAPs. Higher magnifications are shown in the insets. Scale bars: 10 µm (inset, 0.5 µm).