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. 2020 Mar 7;9(3):652. doi: 10.3390/cells9030652

Figure 3.

Figure 3

Notch and Wnt signaling regulate genomic DNA methylation in DPSCs. (A,B): Global DNA methylation levels in DPSCs showing the proportion (%) of 5 mC with respect to total C in control (DMSO, MBIO) with respect to DAPT, BIO, WNT-3A and differentiation induction conditions. Mouse ESCs were used as a control for low DNA methylation. Data are presented as the mean+SEM (n = 8). ##: p < 0.01, ***: p < 0.001; Dunn’s post-hoc test, Kruskal- Wallis H Test. (C): Representative WB showing DNMT1, DNMT3A and DNMT3A expression in DPSCs under different treatments. α-TUBULIN was used as a protein loading control. Mouse ESCs were used as a positive control for DNMT protein expression (right panel). Control on right panel shows untreated DPSCs grown in standard conditions.