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Autophagy has a minor impact on VEGF-induced signaling pathways. (A–G) HUVEC were transfected with control-siRNA or ULK1- plus BECN1-siRNA for 72 h and stimulated with 50 ng/mL VEGF for the indicated times. Cell lysates were subjected to Western blot analyses of the indicated proteins. Representative blots and densitometric evaluations are shown (mean values + SEM, n = 4), * p < 0.05 vs. respective unstimulated control, # p < 0.05 vs. respective samples treated with control-siRNA.
