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. 2020 Mar 12;9(3):694. doi: 10.3390/cells9030694

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Effects of CILK1 variants on phosphorylation of FLAG-kinesin family member 3A (KIF3A) Thr672. GST or GST-CILK1 (wild type (WT); kinase-dead (KD); disease variants) was co-expressed with FLAG-KIF3A in HEK293T cells. Total and phospho-Thr672-specific signals of FLAG-KIF3A were assessed by immunoblotting of anti-FLAG immunoprecipitates with anti-pKIF3A-Thr672 and anti-KIF3A, respectively. (B) Quantification of FLAG-KIF3A-phospho-Thr672 signals normalized against total FLAG-KIF3A signals in (A).