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. 2020 Mar 12;9(3):694. doi: 10.3390/cells9030694

Figure 3.

Figure 3

Effects of CILK1 variants on ciliogenesis. GFP-CILK1 (wild type (WT); kinase-dead (KD); disease variants) were expressed in NIH-3T3 cells. The primary cilium was immunostained with anti-Arl13B. Ciliated cells in both GFP-CILK1-positive and control populations were counted under a confocal immunofluorescence microscopy. Shown is the change in ciliation % of GFP-positive cells (n = 120 cells) relative to control cells (n = 120 cells). Mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, NS = not significant.