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GFP-CILK1 localizes to the base of the primary cilium. NIH-3T3 cells were transfected with GFP-CILK1 and immunostained for the cilium and the centriole with anti-Arl13B and anti-γ-tubulin, respectively. Pseudocolors for GFP-CILK1 (cyan), Arl13B (magenta), and γ-tubulin (yellow) were used to facilitate visualization. Scale bar, 2 µm.
