Figure 5.

CILK1 variants redistribute along the entire axoneme of the primary cilium. NIH-3T3 cells were transiently transfected with GFP-CILK1 (wild type (WT); kinase-dead (KD); disease variants) and immunostained for the cilium with anti-Arl13B. Shown here are (A) the subcellular distribution patterns of GFP-CILK1 WT and disease variants; (B) confocal microscopy images of GFP-CILK1 (cyan), Arl13B (magenta), and their overlay. Pseudocolors were used here to facilitate visualization. Scale bar, 2 µm.