Figure 1.

Cell proliferation. (A) Morphology of bone marrow-derived mesenchymal stem cells (BMSC) and human umbilical vein endothelial cells (HUVEC) cultured in media Complete αMEM and Complete EGM2, respectively, for cell expansion. (B) Morphology of cells in monoculture and coculture in Complete EGM2 medium in the presence/absence of IL-1β or COCl₂ or DMOG, scale bar 50 μm. (C) Proliferation curve of the cells cultured in Complete EGM2 medium supplemented with IL-1β or COCl₂ or DMOG determined by measuring the absorbance of the cells stained with crystal violet at 550 nm wavelength. (D) Relative proliferation of cocultured cells in different media over time compared to day 1. Results are the average + SEM of three experiments performed in quintuplicate from three different cell cultures. Lower chart demonstrates relative proliferation of cocultured cells over time normalized to day 1 (∗∗ p ≤ 0.0018, ∗∗∗ p ≤ 0.0005).