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GB7 cells grown in adherent and as neurospheres. (A) RT-PCR analysis of stemness markers Nestin (NEST), SOX2, CD133, and REST. 18 S was used as a housekeeping gene. (B) Immunostaining for NESTIN, REST and CD133 in GB7 cells grown as a monolayer and as neuropheres in floating conditions (magnification × 100). (C) RT-PCR analysis for gene expression for different genes involved in Notch and EGFR pathways in GB7 and GB8 cells. 18 S was used as a housekeeping gene.
