Figure 5.

Expression of lineage marker genes in the differentiated MSCs. (a) Osteogenic markers, Ocn and Opn, (b) adipogenic markers, Adipoq and Pparg, and (c) chondrogenic markers, Sox9 and Col2a1, were used to determine the multi-lineage differentiation of AT-MSCs and CB-MSCs. MSCs cultured in basic medium without differentiation agents for the same period of time were served as controls. Gene expressions were normalized with housekeeping gene Gapdh. Experiments were performed with three replicates. Data represent mean ± SD; *p < 0.05 and ** p < 0.01.