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. 2020 Mar 17;9(3):737. doi: 10.3390/cells9030737

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of RC-3095 on vascular calcification in HP-fed CKD mouse. CKD mice were fed with a normal phosphate (0.5%, NP) or high phosphate (1.8%, HP) diet. For assessing the effect of RC-3095 treatment, CKD mice were intraperitoneally injected with RC-3095 (1 mg/kg) or vehicle (0.9% saline) 3 times a week for a total 12 weeks. (a and c) Area of aortic calcification in CKD and sham mice was determined by von-Kossa staining. Scale bar: 50 μm. (b) Total RNA was isolated and analyzed by real-time RT-PCR using the specific primers for mouse GRP and GRP-R. The expression level of these genes in the control (untreated) was set to 1 and the values were normalized to the β-actin mRNA levels. * p < 0.05; ** p < 0.01 vs. sham, ^# p < 0.05; ^## p < 0.01vs. NP. (d,e) GRP, GRP-R, Runx2, calponin, Bcl2 and Bad mRNA levels were also examined by real-time RT-PCR. * p <0.05; ** p < 0.01 vs. NP, ^# p < 0.05 vs. vehicle. Data shown are the mean ± SD, obtained from at least three independent experiments.

Effect of RC-3095 on vascular calcification in HP-fed CKD mouse. CKD mice were fed with a normal phosphate (0.5%, NP) or high phosphate (1.8%, HP) diet. For assessing the effect of RC-3095 treatment, CKD mice were intraperitoneally injected with RC-3095 (1 mg/kg) or vehicle (0.9% saline) 3 times a week for a total 12 weeks. (a and c) Area of aortic calcification in CKD and sham mice was determined by von-Kossa staining. Scale bar: 50 μm. (b) Total RNA was isolated and analyzed by real-time RT-PCR using the specific primers for mouse GRP and GRP-R. The expression level of these genes in the control (untreated) was set to 1 and the values were normalized to the β-actin mRNA levels. * p < 0.05; ** p < 0.01 vs. sham, ^# p < 0.05; ^## p < 0.01vs. NP. (d,e) GRP, GRP-R, Runx2, calponin, Bcl2 and Bad mRNA levels were also examined by real-time RT-PCR. * p <0.05; ** p < 0.01 vs. NP, ^# p < 0.05 vs. vehicle. Data shown are the mean ± SD, obtained from at least three independent experiments.