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. 2020 Mar 11;9(3):682. doi: 10.3390/cells9030682

Figure 4.

Figure 4

(a) Cells cultivated on pure NFC scaffold in presence of differentiation factors started to differentiate in 10 days, but they seem to not generate a neural network. Scale bar: 5 µm. (b) Cells cultivated on pure NFC scaffold without differentiation factors did not differentiate. Scale bar: 5 µm. (c) Cells cultivated on NFC/10% CNTs with differentiation factors highly differentiated and generated a very complex neural network, with many connections between neurons. Scale bar: 20 µm. (d) Cells cultivated on NFC/10% CNTs without differentiation factors differentiated and generated a small neural network. Scale bar: 20 µm. (e) Example of synapse found in the sample of cells cultivated in conductive scaffold without differentiation factors. Scale bar: 1 µm.