Table 8.
Function of claudin 14 according to heterologous expression studies in cell lines.
| Claudin | Cell Line | Transfection | TER | PNa/PCl | PNa | PCl | PCa | Ca2+ Flux | Ref. |
|---|---|---|---|---|---|---|---|---|---|
| Human CLDN14 | MDCK II Tet-Off cells | Stable inducible expression | ↗ | ↘ | ↘ | [142] | |||
| Mouse Cldn14 | OK | Stable | ↗ | ↘ | ↘ | NS | ↘∫ ø | [145] | |
| Mouse Cldn14 | MDCK II Tet-Off cells | Stable inducible expression | ↗ | ↘ | ↘ | NS | ↘∫ | [145] |
Relative epithelial permeabilities (e.g., PNa/PCl) are calculated using the Goldman-Hodgkin-Katz equation and the diffusion potential caused by the application of distinct solutions at the apical and basolateral compartment. Absolute permeabilities can be calculated if the transepithelial conductance is known using Kimizuka-Koketsu equation [41]. Flux measurement include trans- and paracellular transport [41]. TER: trans epithelial resistance; NS: not significantly different from control; Px: permeability to ion X; ∫: Transepithelial transport of 45Ca2+ measurement; ø: from apical to basal compartment.