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. 2020 Feb 28;11(3):259. doi: 10.3390/genes11030259

Figure 2.

Figure 2

TDP43 promotes the porcine satellite cells (PSCs) differentiation. (A) Real-time PCR analysis of TDP43 expression in PSCs during the period of proliferation and differentiation. There was a 6h interval between each period. (B) Western blot analysis of TDP43, myogenin (MYOG), myogenic differentiation (MYOD), and myosin heavy chain (MYHC) protein expression levels in PSC in different periods. (C) The knockdown efficiency of TDP43 siRNA. (D) qPCR results showed that the mRNA expression of MYOG, MYOD, and MYHC were significantly decreased by TDP43 knockdown. (E) Western blot results showed that the protein expression of MYOG, MYOD, and MYHC were significantly decreased by TDP43 knockdown. (F) Quantitative analysis of Western blot results. (G) The overexpression efficiency of TDP43-pcDNA3.1. (H) qPCR results showed that TDP43-pcDNA3.1 significantly increased the mRNA expression of MYOG, MYOD, and MYHC. (I) Western blot results showed that TDP43-pcDNA3.1 significantly increased the protein expression of MYOG, MYOD, and MYHC. (J) Quantitative analysis of Western blot results. Mean values ± SD, n = 3. * means p < 0.05, ** means p < 0.01.