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. 2020 Feb 25;11(3):237. doi: 10.3390/genes11030237

Figure 2.

Figure 2

Effect of dsUGT36E1 in the peripheral chemosensory system. A strong expression of neuralized-GAL4 (neur-GAL4) was detected in (A) the antennae and the proboscis, (B) wing margins, (C) legs of adult flies. (D) Antenna stained for Elav protein (red) and anti-GFP (neur-GAL4). (E) Magnified view of antennal neurons expressing Elav and neur-GAL4. (F) Male ability to discriminate sex partners in “neur-GAL4/+, dsUGT36E1/+” testers and in both transgenic controls (neur-GAL4/+ and dsUGT36E1/+) under red light (filled bars) and white light (empty bars). For each test, n > 35. (G) Real-time PCR analysis showing UGT36E1 mRNA level in different tissues of “neur-GAL4/+, dsUGT36E1/+” flies. The significant difference in transcript level ratio is based on a comparison between control (dsUGT36E1/+) and “neur-GAL4/+, dsUGT36E1/+” genotypes (pair-wise fixed reallocation randomization test). For statistics and conditions, see Figure 1. *** p < 0.001; ** p < 0.01; * p < 0.05.