Expression and effect of dsUGT36E1 targeted in various sensory neurons subsets. (A) Targeting the dsUGT36E1 transgene in most olfactory sensory neurons with the Orco-GAL4 transgene (“Orco-GAL4/+, dsUGT36E1/+”) improved mate choice performance compared to both transgenic controls. A similar targeting of this transgene in the P-UGT36E1 mutant background (in “Orco-GAL4/+, dsUGT36E1/+”, but not “P- UGT36E1/+, dsUGT36E1/+”) rescued male performance. For each behavioral test, n > 35. All courtship tests were carried out under red light. (B) Quantitative analysis of UGT36E1 expression in the sensory appendages of “P-UGT36E1/+, Orco-GAL4/+, dsUGT36E1/+” flies compared to “Orco-GAL4/+, dsUGT36E1/+” and “P-UGT36E1/+, dsUGT36E1/+” control flies. The wild-type strain Dijon was used as a reference. For statistics and conditions, see Figure 1. *** p < 0.001; ** p < 0.01; * p < 0.05.