Figure A1.

Yeast wild-type cells transformed with plasmid encoding mitochondrially targeted green fluorescent protein (mt-GFP) and with empty vector ([-]) or plasmids encoding GDAP1 alleles (as indicated) were grown in SC-leu-his medium overnight and were then were shifted to glycerol-containing medium for 4 h to induce expansion of mitochondria. Cells were fixed, stained, and observed using confocal microscopy. GDAP1 was visualized by immunostaining (red), mitochondria using the mt-GFP protein marker (green) and the nucleus was stained with DAPI (blue). Scale bar 5 µm.