. 2020 Mar 12;11(3):300. doi: 10.3390/genes11030300

Table 2.

Description of the primers used for the qPCR analyses with details on the sequences, amplicons’ length, Tm, amplification efficiency and regression coefficient.

Name	Sequence (5’–3’)	Amplicon Length (bp)	Amplicon Tm (°C)	PCR Efficiency (%)	Regression Coefficient (R²)
Actin Fwd	ATGTTGGTGATGAGGCACAG	123	84.4	87.5	0.999
Actin Rev	GCTCGTTGTAGAAGGTGTGATG	123	84.4	87.5	0.999
ApsC Fwd	TCACCGATTCAGGTCTCAAC	148	85.8	87.8	0.998
ApsC Rev	ATGTTGGGGCTCTTGATGTC	148	85.8	87.8	0.998
Cos4 Fwd	GCACACTTCTCCCCAGAGC	104	87.8	89.6	0.999
Cos4 Rev	CCATCGCTTCTCGATATTGG	104	87.8	89.6	0.999
GAPDH Fwd	AGGGCAAACTGAACGGTATC	92	82.9	93.9	0.998
GAPDH Rev	GGCATCGAAAATGGAAGAGC	92	82.9	93.9	0.998
GlkA Fwd	CGCTTGGAACTGCTTTCTTC	106	86.8	93.8	0.995
GlkA Rev	TGTAGGACGATTGGGTTTCG	106	86.8	93.8	0.995
PfkA Fwd	GTTCCCAGCCCAGTTATTTG	93	81.9	91.3	0.998
PfkA Rev	AGCAACAGCGACTTCTTTGG	93	81.9	91.3	0.998
PgiA Fwd	CAACTTCCACCAACTTCTCG	98	82.9	95.0	0.999
PgiA Rev	TTAGCAGACCACCAATGACG	98	82.9	95.0	0.999
SarA Fwd	AGATGCCATTTCCGAGGAC	116	86.4	92.4	0.997
SarA Rev	CCACACTGCACATGAAGACC	116	86.4	92.4	0.997
IsdA Fwd	TCAAGAAGATGTGGCTGTCG	89	85.3	86.0	0.999
IsdA Rev	GATGGTGGGGATGACAATG	89	85.3	86.0	0.999
H2B Fwd	TACAAGGTCCTCAAGCAGGTC	96	83.8	90.6	0.997
H2B Rev	AACACGCTCGAAGATGTCG	96	83.8	90.6	0.997
RS14 Fwd	CACATCACCGATCTTTCTGG	146	88.2	87.0	0.997
RS14 Rev	GTAATGCCGAGTTCCTTGC	146	88.2	87.0	0.997
PTK1 Fwd	TGCTCCTAAACGCAAACTGC	93	84.2	92.0	0.999
PTK1 Rev	CCGTCATGAATCCAGAGTTG	93	84.2	92.0	0.999
CHS1 Fwd	GGACATCAAAAAGGGTGTCG	119	82.2	89.4	0.999
CHS1 Rev	ATGCCTGGAAGAACCATCTG	119	82.2	89.4	0.999
CHS2 Fwd	TCCAAGAGGGTATTGCGAAG	102	82.3	102.0	0.988
CHS2 Rev	TGAATTTGAGGTCCGAGTCC	102	82.3	102.0	0.988
CHS3 Fwd	GCCTGAAGCAAAAGAACAGC	90	83.2	91.4	0.999
CHS3 Rev	AAATGCAGACTTCGGGGTTC	90	83.2	91.4	0.999
CHS4 Fwd	TCATCATCTGCGACGGTATG	115	83.3	94.0	0.996
CHS4 Rev	GAAAATGCCTGAACCTCGTG	115	83.3	94.0	0.996
CHS5 Fwd	CAAGTGCGTTCGTCAACAAG	107	83.4	93.0	0.999
CHS5 Rev	GTCCAAGAAACTCGGCAAAC	107	83.4	93.0	0.999
CHS7 Fwd	CGGAAAAGAACTCGCTCATC	141	85.8	91.2	0.998
CHS7 Rev	GGAAAGCAGAGAATCGCAAC	141	85.8	91.2	0.998
FksA Fwd	AGTTTCTTACGCTGGCAACC	146	89.5	89.0	0.999
FksA Rev	CTTCCTTGGTACAGGGAATCTG	146	89.5	89.0	0.999
EngA Fwd	TCAAGTGTGGAAGGGTATCG	107	84.8	93.3	0.994
EngA Rev	AGCCGTAATGGAAGTGATGG	107	84.8	93.3	0.994
ExgB Fwd	TGGATGATGGGAGATGAGTG	90	82.0	102.8	0.996
ExgB Rev	GGCCTTTGTTTGACCAAGTG	90	82.0	102.8	0.996
ExgC Fwd	TAAACACAGGCGGATGGTTC	145	88.1	91.3	0.994
ExgC Rev	CACGAATTCCAGTGGTCTTG	145	88.1	91.3	0.994
ExgD Fwd	GACGCAAACGAAGAGAATCC	101	86.3	88.2	0.998
ExgD Rev	TGGTAGTAAATCGCCCTGTG	101	86.3	88.2	0.998
celA Fwd	CATTACCGCCCTTTTGTCAC	117	81.0	88.7	0.998
celA Rev	ATGAAGAACCACGCAAGACC	117	81.0	88.7	0.998