Figure 4.

The mRNAsi index associated weighted gene co-expression network analysis (WGCNA) of lung adenocarcinoma. (a,b) differentially expressed genes; green indicates downregulated genes and red indicates upregulated genes. The Wilcox test shows that there are 500 genes downregulated and 2876 genes upregulated, false discovery rate (FDR) < 0.05 and |logFC| > 1 are considered meaningful. (c) The samples with a deflection of gene expression were cut off, and the appropriate power value was determined (=4) as a soft threshold, taking both the scale-free correlation coefficient and mean connectivity into consideration. Then, a co-expression module in LUAD was identified using the power value (Appendix A). Finally, the dendrogram was drawn with branches of the cluster corresponding to the 17 different gene modules, and modules with a standard of the minimum size of 50 genes for module merging were screened out; (d) the correlation between the gene modules and clinical traits, including both mRNAsi and EREG-mRNAsi. The correlation coefficient in the heat-map is equal to the correlation between each gene module and the clinical traits, which decreased from red to blue, also annotated with the corresponding p-value.