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. 2020 Mar 13;11(3):306. doi: 10.3390/genes11030306

Figure 9.

Figure 9

Effect of heat stress (HS) on the mRNA and protein expression of Hspa1b, ATP5f1 and Inmt in rats. (A) The mRNA expression levels of Hspa1b, ATP5f1, and Inmt in liver (in vivo). The expression levels of Hspa1b, ATP5f1, and Inmt in the liver tissue of H120-treated rats and the time-matched control group (n = 5); (B) regression analysis of the top 3 significant DEG expression levels (x-axis) in the liver and biochemical indicator levels of significant changes in serum (y-axis); (C) the expression level of Hspa1b, ATP5f1, and Inmt in BRL cell (in vitro). The control BRL cells were cultured at 37 °C and the HS-cells were treated under H30, H60, and H120 conditions. Three independent experiments were performed for each treatment; (D) the effect of HS on the expression of Hspa1b, ATP5f1, and Inmt proteins in BRL cells by WB and their expression relative to GAPDH. All data are presented as mean ± SEM. Each bar indicates the standard error of means. p < 0.05 is a significant regression. * represents p < 0.05 and ** represents p < 0.01.