Enzyme activity of recombinant FPPS homolog (LeGPPS) from L. erythrorhizon. The enzyme reaction products were analyzed by GC-MS after dephosphorylation by acid/alkaline phosphatases. A, Total ion chromatograms of reaction products of recombinant LeGPPS with DMAPP, GPP, or FPP as acceptor substrates in the presence of IPP, the donor substrate. The prenyl acceptor substrates GPP and FPP for FPPS and GGPPS, respectively (bottom two chromatograms), were detected in their respective assay mixtures. B, Mass fragmentation patterns of a standard specimen of geraniol and the enzyme reaction product of recombinant LeGPPS with IPP and DMAPP as substrates.