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Biochemical assays of recombinant LeGPPS-H100L and LeFPPS1-L100H. The enzyme reaction products were analyzed by GC-MS after dephosphorylation by acid/alkaline phosphatases. Total ion chromatograms of reaction products are shown for LeGPPS-H100L and LeFPPS1-L100H using IPP as donor substrate and DMAPP, GPP, and FPP as acceptor substrates.
