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The enzyme reaction products were quantitated by UPLC-MS analysis. Data are shown as means ± SD of three technical replicates.
| Substrate | LeGPPS | ||
|---|---|---|---|
| Km (µm) | kcat (min−1) | kcat/Km (μm−1⋅min−1) | |
| DMAPP (at 100 µm IPP) | 8.17 ± 1.35 | 2.24 ± 0.15 | 0.27 ± 0.03 |
| GPP (at 100 µm IPP) | – | – | – |
| IPP (at 60 µm DMAPP) | 2.69 ± 0.60 | 2.83 ± 0.46 | 1.06 ± 0.08 |
