Table 2. Kinetic Parameters for LeFPPS1.

The enzyme reaction products were quantitated by UPLC-MS analysis. Data are shown as means ± SD of three technical replicates. Asterisk indicates the value calculated from near detection limit of UPLC-MS analysis because of the high affinity of LeFPPS1 to GPP.

Substrate	LeFPPS1
	Km (µm)	kcat (min−1)	kcat/Km (μm−1⋅min−1)
DMAPP (at 100 µm IPP)	2.20 ± 0.48	3.48 ± 0.21	1.58 ± 0.25
GPP (at 100 µm IPP)	0.10 ± 0.03*	2.49 ± 0.04	26.14 ± 9.65
IPP (at 60 µm DMAPP)	3.03 ± 0.91	3.90 ± 0.67	1.29 ± 0.16