Figure 1.

MiR159 and GAMYB homologs in tobacco. A, Sequence alignments of tobacco mature miR159 and the miR159 binding sites in NtGAMYB transcripts. Underlined nucleotides represent the cleavage site, lowercase gray letters represent mismatches to Nt-miR159, and G:U pairing is shown in uppercase gray letters. B, RT-qPCR measurement of miR159 and GAMYB transcript levels in tobacco. RNA was extracted from pools of tissue from two plants, each composed of two young leaves (L) or five mature open flowers (F). The mRNA levels of the NtGAMYB genes were normalized to PROTEIN PHOSPHATASE2A and the miR159 RNA levels were normalized to snoR101. Measurements are the average of three biological replicates, with error bars representing the se.