Figure 2.

Expression of MIM159 in transgenic tobacco results in strong phenotypic defects. A, Multiple T1 independent MIM159 transgenic lines (L2, L4, and L8) displayed smaller growth stature compared to the wild type (WT). B, Eight-week-old wild-type and MIM159 transgenic tobacco grown under identical conditions. C, Close-up view of the MIM159 transgenic plant depicted in B. Arrows indicate sectorized chlorosis on leaves. D, Young leaf of 8-week-old wild-type and MIM159 transgenic plants. E, MIM159 plants produce smaller flowers, paler petals, and shorter anther filaments compared to wild-type plants. In D and E, the various organs were digitally extracted for comparison. F, Flowering time of wild-type and MIM159 transgenic plants. All plants were grown without antibiotic selection. The bar charts represent the mean of flowering time of eight wild-type plants and 13 MIM159 plants from three independent lines. Error bars represent the se, and the asterisk indicates statistically significant difference determined by Student’s t test. G, Seed weight of wild-type and MIM159 transgenic plants. The bar charts represent the average seed weight of >100 seeds from five wild-type and five MIM159 plants from three independent lines. Error bars represent the se, and the asterisk indicates statistically significant difference determined by Student’s t test.