Transcript profiling of MIM159 transgenic rice. A, RT-qPCR measurement of MIM159, OsGAMYB, and OsGAMYBL1 expression. RNA was extracted from wild-type (WT, 2–3 lines), VC (2–3 lines) and MIM159 (4–6 transgenic lines) lines, with analyzed samples comprising three flag leaves (Leaves) or 30 florets (Flowers) and RNA levels normalized to ACTIN. B, MiR159 levels in a subset of the lines in A. RNA levels were normalized to snoR14. Measurements are the average of three technical replicates.